Amapayipi ngokuvamile asetshenziswa kumalabhorethri ukudlulisa umthamo wamamililitha oketshezi, ukusuka kobuncane obungu-1 ml ukuya ku-50 ml. Izitsha zingalahlwa kupulasitiki oyinyumba noma ziphinde zisetshenziswe engilazini ekwazi ukuvuleka. Womabili ama-pipettes asebenzisa i-pipette ukuze afune futhi axoshe uketshezi. Amasayizi ahlukene ama-pipettes asetshenziswa ekuhlolweni okuhlukene nge-pipette efanayo. Isibonelo, ama-pipette abalulekile ekuxubeni izixazululo zamakhemikhali noma ukumiswa kwamaseli, ukudlulisa uketshezi phakathi kweziqukathi ezihlukene, noma ama-reagents okucwenga ezindaweni ezihlukene. Uma nje ukunakekelwa ngokucophelela kukhokhwa kumthamo we-liquid aspirated futhi exoshwa, ama-pipettes angaba ithuluzi eliwusizo elabhorethri yokudlulisa ngokunembile imiqulu ye-milliliter yamanzi.
Izinhlobo zama-pipettes kanye nezingxenye eziyisisekelo zamapayipi
Amapayipi ngokuvamile amashubhu epulasitiki ayinyumba asetshenziswa kanye; zingaphinde zibe amashubhu engilazi asebenziseka kalula, asebenziseka kabusha.
Wonke ama-pipettes asebenzisa i-pipette lapho efaka ipayipi.
I-pipette iqeda isidingo sokuthi abacwaningi bafake i-pipette ngomlomo njengangaphambili. Leyo ndlela yakudala yamapayipi ayinconywa ngoba inamandla okubangela imiphumela emibi ekumunceni uketshezi emlonyeni.
Ibhola le-pipette liwuhlobo lwe-pipette ngokunemba okubi kakhulu. Ngokuvamile kuhlanganiswe ne-pipette yengilazi ukudlulisa inani eliguquguqukayo loketshezi.
Amaphampu e-pipette nawo afaneleka ama-pipettes engilazi, angadlulisela imiqulu ye-liquid enembe kakhudlwana. Amaphampu e-pipette ngokuvamile afaneleka ukusabalalisa umthamo ofanayo woketshezi ngokuphindaphindiwe.
Ama-pipettes omsizi ama-pipettes avame kakhulu. Iqukethe izingxenye eziningana eziyinhloko: umlomo lapho i-pipette ifakwe khona futhi lapho i-membrane yokuhlunga ifakwe khona, evikela ingaphakathi le-pipette yomsizi ekungcolisweni koketshezi.
Izinkinobho ezimbili zingabonakala kwisibambo se-pipette yomsizi. Lapho inkinobho engenhla icindezelwa, uketshezi luyafiswa, futhi lapho inkinobho engezansi icindezelwa, uketshezi luyakhishwa.
Amapayipi amaningi omsizi nawo ane-knob yokulawula izinga lokukhipha uketshezi. Isibonelo, ingasethwa ukuze ikhulule uketshezi ngaphansi kwengcindezi, noma ingasethelwa ukukhululwa kwamandla adonsela phansi ngaphandle kwamandla angaphandle.
Nakuba amanye amapayipi omsizi eza nentambo yamandla, amaningi asebenza ngebhethri.
Amanye ama-pipettes omsizi afika nokuma okuhambisana nendawo yesibambo, okuvumela ukuthi i-pipette yomsizi ibekwe eceleni kwayo uma ingasetshenziswa ngaphandle kokukhipha i-pipette.
Njengoba kushiwo ngaphambili, i-pipette efanayo ingasebenzisa amasayizi ahlukene we-pipettes kuye ngevolumu okufanele ifakwe ngamapayipi, kusukela ku-0.1 milliliters kuya kumashumi amamililitha.
Ukusebenza okuyisisekelo kwama-pipettes
Okokuqala, khetha i-pipette yesayizi efanele ngokusekelwe kumthamo we-liquid ofuna ukuyidlulisela. Bese uvula iphakheji kusuka phezulu, thinta kuphela ingxenye engaphezu kophawu lokumaka, uyifake esihlokweni se-pipette, bese ususa iphakheji esele.
Okulandelayo, thatha i-pipette ngesandla esisodwa bese uvula isembozo sesitsha esiqukethe uketshezi ofuna ukulufisa. Ugcina i-pipette imile, cindezela ngobumnene inkinobho engaphezulu ukuze ulangazelele kancane isampula yakho.
Sebenzisa umugqa othweswe iziqu odongeni lwe-pipette ukuze ulinganise umthamo woketshezi ofuna ukuwudlulisela. Qaphela ukuthi ivolumu kufanele ifundwe phansi kwe-meniscus, hhayi phezulu.
Bese udedela ngokucophelela uketshezi esitsheni osithandayo, uqaphele ukuthi ungavumeli ithiphu le-pipette lithinte noma iyiphi indawo engeyona oyinyumba.
Sebenzisa ukuqapha namandla amnene lapho ukhipha uketshezi, ikakhulukazi uma usebenzisa amapayipi umthamo wevolumu encane, ukuze ugweme ukungcolisa isihlungi se-pipette nesampula, noma ukulimaza i-pipette yomsizi. Ukuphatha kabi lapho usebenzisa i-pipette yomsizi kungacasula abanye abantu abanolwazi kakhudlwana elebhu, okungenzeka kudingeke bahlukanise i-pipette ukuze balungiswe. Lapho umpompa inani elikhulu loketshezi noma uketshezi olukhiphayo, isivinini sokudlulisa uketshezi singanyuswa ngokucindezela inkinobho kanzima.
Okokugcina, khumbula ukulahla kahle utshani ngemva kokudlulisa uketshezi.
Isicelo
Manje njengoba usukwazi ukusebenzisa i-pipette, ake sibheke ngokuningiliziwe kwezinye izinhlelo zokusebenza zaselabhorethri ezivamile.
Isinyathelo esibalulekile lapho culturing futhi Plating amaseli ukusatshalaliswa okufanayo kwamaseli isisombululo sokugcina. Ukumiswa kwamaseli kungaxutshwa ngobumnene nangempumelelo usebenzisa i-pipette, ehlanganisa ngesikhathi esisodwa izixazululo zamakhemikhali nama-reagents.
Ngemva kokuhlukaniswa noma ukucutshungulwa kwamaseli okuhlola, ama-pipette angasetshenziswa ukudlulisa ama-clone amaseli wonke ukuze anwetshwe noma ukuhlaziya kokuhlolwa okulandelayo.
Isikhathi sokuthumela: Aug-31-2022