I-glassware entsha noma esetshenzisiwe kufanele iqale ifakwe emanzini ukuze kuthambise futhi kuncibilikise izinto. I-glassware entsha kufanele ivele igezwe ngamanzi kampompi ngaphambi kokusetshenziswa, bese icwiliswa ubusuku bonke nge-5% hydrochloric acid; I-glassware esetshenzisiwe ivame ukunamathiselwa nenani elikhulu lamaprotheni namafutha, yomile ngemva kokuba akulula ukuyikhuhla, ngakho kufanele ifakwe ngokushesha emanzini ahlanzekile ukuze ikhuhle.
1. Izindaba ezidinga ukunakwa:
Ngemuva kokuhlanza nokukhipha amagciwane ngaphambi kokusetshenziswa, kungakhathaliseki ukuthi isitsha se-petri sihlanzekile noma cha sinomthelela omkhulu emsebenzini, singathinta i-ph ye-culture medium, uma kukhona amakhemikhali athile, azovimbela ukukhula kwamagciwane.
Izitsha ze-petri ezisanda kuthengwa kufanele zihlanjululwe ngamanzi ashisayo kuqala, bese zicwiliswa ku-hydrochloric acid isisombululo ngengxenye enkulu ye-1% noma i-2% amahora ambalwa ukuze kukhishwe izinto zamahhala ze-alkaline, bese zihlanjululwa ngamanzi acwecwe kabili.
Uma ufuna ukukhiqiza amabhaktheriya, bese usebenzisa umusi wokucindezela okuphezulu (okujwayelekile 6.8 * 10 5 Pa high pressure steam), inzalo ku-120 ℃ for 30min, owomile ekamelweni lokushisa, noma inzalo yokushisa eyomile, ukubeka isitsha se-petri kuhhavini. , ukulawula izinga lokushisa cishe ku-120 ℃ ngaphansi kwesimo se-2h, ungabulala izinyo lebhaktheriya.
Izitsha ze-petri ezifakwe inzalo zingasetshenziselwa ukujova kanye nesiko kuphela.
2. Sebenzisa indlela:
Beka ibhodlela le-reagent ukuze lisetshenziswe endaweni efanele endaweni yokusebenza, futhi ukhulule ikepisi lebhodlela le-reagent elizosetshenziswa.
Beka izitsha ze-petri phakathi nendawo yakho yokusebenza;
Susa i-cap yebhodlela le-reagent bese uphonsa i-reagent ebhodleleni le-reagent nge-pipette.
Beka isivalo sesitsha se-petri ngemuva kwaso;
Faka ngobumnene i-culture medium ngqo esisekelweni sohlangothi olulodwa lwesitsha;
Faka isivalo esitsheni se-petri;
Beka isitsha ohlangothini lwaso, uqaphele ukuthi ungavumeli okuphakathi kungene endaweni encane phakathi kwesivalo nangaphansi;
Susa utshani obusetshenzisiwe.
Isikhathi sokuthumela: Apr-26-2022