Isitsha se-petri siwumkhumbi ojwayelekile waselabhorethri, ohlanganisa phansi okumise okwe-disc nesembozo, ikakhulukazi esenziwe ngepulasitiki nengilazi, futhi ingilazi ingasetshenziselwa izinto zezitshalo, isiko le-microbial kanye nesiko lokunamathela kwamangqamuzana ezilwane. Iningi lepulasitiki liyalahlwa, lilungele ukujova elabhorethri, i-streaking, kanye nokuhlukaniswa kwamagciwane ukuze kutshalwe izinto zezitshalo.
Indlela/isinyathelo:
1
Izitsha ze-Petri zivame ukwakhiwa ngendlela eqinile yesiko lamapuleti (okungukuthi umsuka wegama lepuleti lepuleti). Ukukhiqizwa kwe-plate medium ukuhlakaza i-agar medium efakwe inzalo ngamanzi afudumele (oyinyumba), susa ipulaki likakotini lokuhlola, Dlulisa umlomo weshubhu phezu kwelangabi lesibani sotshwala, bese uvule kancane isivalo se-sterilized. isiko dish, ukuze umlomo we-tube yokuhlola ukwazi ukujula. Isatshalaliswa ngokulinganayo phansi kwesitsha futhi ifinyezwe ukuze kutholwe isiko lepuleti.
2
Ngenxa yokuthi ukukhiqizwa, ukuthuthukiswa kanye nokukhula kwamabhaktheriya kuhlobene ngokuqondile nendawo enikeziwe (ukudla okunomsoco), ikakhulukazi ukuhlolwa nokuhlaziywa komthamo, kunokubaluleka okubalulekile kwenani lemisoco enikeziwe.
3
Inani lokudla okunomsoco elinikezwa ngesikhathi sesiko lebhaktheriya, noma ngabe lifana, nokuthi ingabe iphansi lesitsha se-petri liyisicaba kubaluleke kakhulu. Uma phansi kwesitsha se-petri kungalingani, ukusatshalaliswa kwe-agar medium kuzohluka kuye ngokuthi i-bottom dish ye-petri iyisicaba noma cha. Ukunikezwa akwanele, okuhlobene eduze nokuhlaziywa kobuningi, ngakho-ke i-quantitative petri dish iyadingeka ukuthi ibe sicaba ikakhulukazi ngenxa yesizathu. Kodwa-ke, ukuze kubonakale izici ezijwayelekile (ukuhlolwa kwamagciwane, ukukhula kwekoloni, ukuzala, njll.), izitsha ze-petri ezijwayelekile zingasetshenziswa.
Izinyathelo zokuzivikela
Ngemuva kokuhlanza nokukhipha amagciwane ngaphambi kokusetshenziswa, kungakhathaliseki ukuthi isitsha se-petri sihlanzekile noma cha sinethonya elikhulu emsebenzini, elingathinta i-pH yendawo. Uma kukhona amakhemikhali athile, azovimbela ukukhula kwamagciwane.
Isikhathi sokuthumela: Nov-22-2022