Izindaba

1 Indlela ye-smear iyindlela yokwenza ifilimu efaka izinto ngendlela efanayo ku-aingilazi slide. Izinto ze-smear zihlanganisa izinto eziphilayo ezinengqamuzana elilodwa, ulwelwe oluncane, igazi, uketshezi lwe-bacterial culture, izicubu ezixegayo zezilwane nezitshalo, amasende, ama-anther, njll.
Naka lapho ugcoba:
(1) Isilayidi sengilazi kufanele sibeihlanzekile.
(2) Isilayidi sengilazi kufanele sibe sicaba.
(3) Ukugqoka kufanele kube iyunifomu. I-smear liquid yehliselwa kwesokudla phakathi kwe-slide, futhi isakazwe ngokulinganayo nge-scalpel blade noma i-toothpick.
(4) Ukugqoka kufanele kube mncane. Sebenzisa esinye isilayidi njengesiphusha, bese uphusha ngobumnene usuka kwesokudla uye kwesokunxele eduze kwendawo ye-slide lapho kuconselwa khona isisombululo se-smear (i-engeli ephakathi kwamaslayidi amabili kufanele ibe ngu-30°-45°), bese usebenzisa ungqimba oluncane ngokulinganayo.
(5) Kulungisiwe. Ukulungisa, indlela yokulungisa amakhemikhali noma eyomile (amagciwane) ingasetshenziselwa ukulungisa.
(6) Ukudaya. I-methylene eluhlaza okwesibhakabhaka isetshenziselwa amagciwane, ibala likaWright lisetshenziselwa igazi, futhi ngezinye izikhathi iodine ingasetshenziswa. Isixazululo sokudaya kufanele simboze yonke indawo ependiwe.
(7) Hlanza. Gcoba ngephepha elimuncayo noma uthose owomile.
(8) Vala ifilimu. Ukuze uthole isitoreji sesikhathi eside, vala amaslayidi nge-Canadian gum.
2. Indlela yethebhulethi iyindlela yokwenza amashidi ngokubeka izinto zebhayoloji phakathi kwesilayidi sengilazi nesiliphu sekhava bese usebenzisa ukucindezela okuthile ukuze kuhlakazwe amaseli ezicubu.
3. Indlela yokukhweza yindlela lapho izinto zebhayoloji zivalwa sezizonke ukuze kwenziwe izifanekiso zama-slide. Le ndlela ingasetshenziswa ukwenza izikhwebu zesikhashana noma ezihlala njalo. Izinto zokulayisha izingcezu zifaka: izinto eziphilayo ezincane ezifana ne-Chlamydomonas, i-Spirogyra, i-Amoeba, ne-nematodes; I-Hydra, i-epidermis yeqabunga yezitshalo; amaphiko, izinyawo, izingxenye zomlomo wezinambuzane, amangqamuzana e-epithelial omlomo womuntu, njll.
Kufanele kuqashelwe ukulungiswa kwendlela yama-slide:
(1) Lapho ubambe i-slide, kufanele sibe sicaba noma sibekwe endaweni yesikhulumi. Lapho udonsa amanzi, inani lamanzi kufanele lifanele, ukuze livele lihlanganiswe ingilazi yekhava.
(2) Impahla kufanele yembulwe ngenaliti yokuhlakaza noma ama-tweezers ngaphandle kokugqagqana, futhi yenziwe isicaba endizeni efanayo.
(3) Lapho ubeka ingilazi yekhava, vala kancane kancane ithonsi lamanzi ukusuka ohlangothini olulodwa ukuze uvimbele ukuvela kwamabhamuza omoya.
(4) Uma ugcoba, faka ithonsi lesisombululo sombala ngakolunye uhlangothiikhava ingilazi, bese uyidonsa ngakolunye uhlangothi ngephepha elimuncayo ukuze wenze isifanekiso esingaphansi kwengilazi yekhava sibe nombala olinganayo. Ngemva kombala, sebenzisa indlela efanayo, yehlisa iconsi lamanzi, ncela isixazululo esingcolisayo, bese ubheka ngesibonakhulu.